Radioimmunoassay of Human Serum Thyrotrophin
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay of human serum thyrotrophin.
The double antibody radioimmunoassay of serum thyroid-stimulating hormone (TSH) allows measurement of circulating levels of the hormone in most normal subjects. The serum TSH level in normal subjects is 1.6 +/- 0.8muU/ml. Patients with non-toxic goitre and acromegaly have normal TSH levels. Values are always raised in hypothyroid patients (with primary thyroid disease) and are significantly low...
متن کاملTechnical aspects of the radioimmunoassay of thyrotrophin.
There are two main animal sources; rabbit antihuman TSH available from the National Pituitary Agency (NPA) or the Medical Research Council (MRC) and guinea-pig antihuman TSH prepared locally. Franchimont (1972) has also reported the use of antibodies to porcine TSH which crossreact with human TSH but this reagent is not generally available. Locally produced guinea-pig anti-TSH is used at a fina...
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A new radioimmunoassay for human parathyroid hormone (PTH) in serum, which can measure the hormone present in 94% of the normal sera tested, is described. It is based on the ability of human PTH to compete with (131)I-labeled bovine PTH for binding to an antiserum directed against porcine PTH. This antiserum distinguishes between human PTH extracted from parathyroid adenomata and that present i...
متن کاملRadioimmunoassay of triiodothyronine in unextracted human serum.
Serum triiodothyronine (T-3) concentrations have been estimated by radioimmunoassay using unextracted serum. The serum T-3 concentrations have been shown to be similar in two separate European populations (0.76-1.67 ng/ml). Raised T-3 values have been observed in all subjects with hyperthyroidism. Low values are seen in hypothyroidism although there is some overlap with the normal range. There ...
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Radioimmunoassay of serum estrogen (sum of estrone and estradiol-17f3) in 0.5 ml of human serum is described. After dilution of the serum, the estrogens are adsorbed on a Sephadex G-15 column in the presence of dilute HC1 and then eluted with benzene. After evaporation of the benzene eluate, the estrogens are dissolved in bovine serum albumin (5 g/. liter solution, at pH 8.0). An aliquot of thi...
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ژورنال
عنوان ژورنال: BMJ
سال: 1971
ISSN: 0959-8138,1468-5833
DOI: 10.1136/bmj.1.5749.582